Le Thi Kim Tuyen | Thang Long Journal of Science: Mathematics and Mathematical Sciences

Research interests

Restriction enzyme

Methyltransferase

Molecular biology

Protein-ADN specific interactions - Gene cloning

Environmental bacteria

Bioinformatics

Education

2015: Massive Open Online Courses (MOOC) Bioinformatic Methods I and II.1970 : Baccalauréat, Paris, France, section C, Mathématiques-physiques.

2008: Master in Philosophy: Community and individual identity: Psychoanalysis approach. Paul-Valéry Montpellier III university, France.

1999: PhD in Medecine, Vietnam. Thesis title: Screening of restriction enzymes in natural bacteria applied to molecular epidemiology methods.

1984: General Immunology courses, Institut Pasteur, Paris.

1980: PhD in bacteria genetics. Thesis title: Contribution to transformation study in Pseudomonas solanacearum.

1976: Molecular genetics certificate, Aix-Marseille university.

1975: Master in Plant breeding, Paris XI university. Thesis title: In vitro regeneration study of Glycine max

1974: Biochemistry and Genetics Degree, Paris VI-VII university, France

1972: DUES (Diplôme Universitaire d’Etudes Supérieures) Chemistry Biology, Mathematics et Physics Section, Paris VII university, France.

Honors and awards

Fellow, The Third World Academy of Science, elected 2004.

Publications

1. Le T.K.T., Leccas D. And Boucher C. (1978). Transformation of Pseudomo-

nas solanacearum strain K60. Proc. 4th Int. Conf. Plant Path. Bact., Angers: 819-822.
2. Zischek C., Le T.K.T., Boistard P., and Boucher C. (1981). Presence of a megaplasmid in strains of Pseudomonas solanacearum. Proc. 5th Int. Conf. Plant Path. Bact., California: 427-437.

3. Maina C.V., Grandea III A.G., Le T.K.T., Asikin N., Williams S.A. and Mc Reynolds L. (1987). Difilaria immitis genomic complexity and characteriza- tion of a structural gene . In “Molecular Paradigms for eradicating helminthic parasites” ( Mac Innis A.J. ed.), Alan R. Liss Inc., New York : 193-204

4. Grandea III A.G., Le T.K.T., Asikin N., Davis T.B., Philipp M., Cohen C. and Mc Reynolds L. (1989). A -gt11 cDNA recombinant that encodes Difilaria immitis paramyosin. Mol. Biochem. Parasitol., 35: 31-42.
5. Le Thi Kim Tuyen, Duong Thi Luu, Nguyen Thuy Loan, Nguyen Le Trang (1990). An early pregnancy test. (In vietnamese). Scientific reports of the National Institute of Hygiene and Epidemiology, Hanoi

6. Nguyen Thi Van Anh, Le Thi Kim Tuyen, Le Minh Nguyet, Le Dinh Luong (1993). An investigation toward the discovery of restriction enzymes in several microorganisms in Vietnam. J. Genetics and its applications, 3: 20-24 (in vietnamese).

7. Le Thi Kim Tuyen, Nguyen Thu Van, Hoang Thuy Nguyen (1995). Identification of poliovirus strains by PCR-RFLP. (In vietnamese). J. Epidemiology, Vietnam, V, 2(20): 10-15
8. Thierry D., Chavarot P., Marchal G., K.T. Le Thi, M.L. Ho, N.L. Nguyen, N.V. le, S. Ledru, Fumoux F., Guesdon J.L. (1995). Mycobacterium tuberculosis strains unindentified using the IS6110 probe can be detected by oligonucle- otides derived from the Mt308 sequence. Res. Microbiol., 146: 325-328.

9. Le Thi Kim Tuyen, Thierry D., Ho Minh Ly, Nguyen Thuy Loan, Chavarot P., Nguyen Ngoc lan, Le Ngoc Van, Marchal G., Dang Duc Trach, Guesdon J.L. (1995). Investigation for using IS6110 in diagnosis and identification of Mycobacterium tuberculosis strains isolated in Vietnam.(in Vietnamese). The year of Louis Pasteur Sub-Regional Symposium, October, 1995, Hanoi: 125-130.

10. Le Thi Kim Tuyen (1995). Molecular biology methods applied to medical sciences. (in Vietnamese). In “Workshop in Biochemistry”, Vol. IV, Medical Publishing House: 32-41.
11. Le Thi Kim Tuyen, Vu Thi Kim Lien, Vu Hong Nga (1996). Screening of restriction enzymes in natural bacteria isolated in Vietnam. (in vietnamese). Symposium in Microbiology, Epidemiology and Immunology, January 1996, Ho Chi Minh Ville: 112-116.

12. Nguyen thi Hien Thanh, Le Thi Kim Tuyen, Pham Ngoc Oanh, Huynh Phuong Lien (1996). Characterization of the poliovirus strains isolated in 1994 from acute flaccid paralysis patients in the North of Vietnam. (In Vietnamese). Symposium in Microbiology, Epidemiology and Immunology, January 1996, Ho Chi Minh Ville : 21-24.

13. Le Thi Kim Tuyen, Vu Hong Nga (1997). Determination of Bci VI, a new restriction enzym isolated from Bacillus circulans. J. Preventive Medecine, VII, 4 (34) : 5-10.
14. Le Thi Kim Tuyen, Vu Thi Kim Lien (1997). Determination of Sml I, a new restriction enzym isolated from Stenotrophomonas maltophilia. J. Preventive Medecine, VII, 4 (34) : 11-16.

15. Situation of tuberculosis in Vietnam from an IS 6110 fingerprint analysis of Mycobacterium tuberculosis strains isolated in patients. (in Vietnamese). J. Preventive Medecine, VIII, 2 (36) : 5-13.

16. Lê Thi Kim Tuyen. (1998). Recherche d’enzymes de restriction pour le développement de méthodes basées sur la RFLP : exemple d’application à l’identification du poliovirus par PCR-RFLP. Revue Médicale, 2 : 113.
17. Le Thi Kim Tuyen and Nguyen Van Hau (1998). Local screening of restriction enzymes and RFLP-based diagnostic : application for the porcine stress syndrome diagnosis. In " Scientific reports of Vietnam-India seminar on Biotechnology, 11/1998, Ministry of Science, Technology and Environment, Institute of Agricultural Genetics: 1-4.

18. Le Thi Kim Tuyen, Nguyen Thu Van, Hoang Thuy Nguyen (1998). Identification of poliovirus strains by PCR-RFLP. In "Research works in 1995-1996", Medical Publishing House, Vietnam: 40-42.
19. Purification of viral RNA from faecal specimens for rotavirus detection and characterisation in Vietnam (1998). In " Scientific reports of Vietnam-In- dia seminar on Biotechnology, 11/1998, Ministry of Science, Technology and Environment, Institute of Agricultural Genetics: 1-10.

20. Nguyen Van Trang, Nguyen Hanh Phuc, Le Thi Kim Tuyen, Huynh Phuong Lien, Nguyen Van Man et Gass I.R. (1998). Etude préliminaire sur l’infection de rotavirus chez des enfants atteints d’entérites gastriques aigües, au Vietnam. Revue Médicale, 2 : 114.

21. Le Thi Kim Tuyen, Vu Thi Kim Lien, Pham Van Ty (1998). Determination of restriction enzymes of Streptomyces strains isolated from soil samples nearby Hanoi. Vietnamese J. Sci. Technol., XXXVI, 6B: 20-24.
22. Le Thi Kim Tuyen (1999). Evaluation on the screening of restriction enzymes isolated from natural bacteria in Vietnam. Proceedings, National biotechnology conference, Hanoi 1999 : 1329-1334. Science and Technology Editions.

23. Tuyen L.T.K., B. K. Hoa, H.M. Ly, L. N. Van, N.T.N. Lan, D. Chevrier and J.-L. Guesdon. (2000). Molecular fingerprinting of Mycobacterium tuberculosis strains isolated in Vietnam using IS6110 as probe. Tubercle and Lung Disease, 80(2): 75-83.

24. Le Nhat Minh, Le Thi Kim Tuyen, Vu Thi Kim Lien (2001). Using lysozyme to break bacteria in screening restriction enzyme. (in Vietnamese). J. Genetics and Applications, 1/2001: 16-21.

25. Nguyen Hoai Giang, Nguyen Hanh Phuc, Le Thi Kim Tuyen (2001).

Optimization of PCR for amplification of D1S80 (pMCT118) alleles by using Vent ADN polymerase. (in vietnamese). J. Med. Res., 16, 3: 20-23.
26. Nguyen Hoai Giang, Nguyen Hanh Phuc, Le Thi Kim Tuyen. Applying of Vent DNA polymerase for amplification of exon 56 (dystrophin gene) by PCR. (in vietnamese). J. Genet. Applic., 2/2001, 28-30.

27. Nguyen Hoai Giang, Nguyen Hanh Phuc, Le Thi Kim Tuyen (2002). Applying of Vent DNA polymerase for the amplification of D17S5 (YNZ22) alleles by PCR. (in Vietnamese). J. Biol., 24(2): 56-58.
28. Le Thi Kim Tuyen (2003). Nucleotide analysis of restricted fragment DNA extremities for characterizing the restriction enzyme cutting activity. (in Vietnamese). J. Medical Research, 23, 3: 105-108.

29. Le Nhat Minh, Le Thi Kim Tuyen, Do Trung Phan, Vu Minh Chau (2003). Detection of Haemophilia A carrier by PCR-RFLP in the intron 18 region. (in vietnamese). Journal of Practical Medecine, 8(458): 58-60.
30. Le Thi Kim Tuyen, Hoang Lan, Vu Thi Kim Lien (2003). DNA cut site determination for new restriction enzymes. (in vietnamese). In National Biotechnology Conference 2003, Science and Techniques Publishing house, Hanoi: 1151-1154.

31. Le Nhat Minh, Le Thi Kim Tuyen, Do Trung Phan, Vu Minh Chau and Vo Thuong Lan (2003). (in vietnamese). Carrier detection of Hemophilia by PCR-RFLP analysis of the Bcl I/intron 18 DNA polymorphismes. In " The National Biotechnology Conference 2003", Science and Techniques Publishing House, Hanoi: 1025-1028.

32. Le Thi Kim Tuyen (2003). Nucleotid analysis of restricted fragment DNA extremities for characterizing the restriction enzyme cutting activity. (In vietnamese). J. Med. Res., 23(3): 106-109.
33. Halle L., Bach K.H., Martageix C., Bianchi F., Le T.Kim T., Morel-Kopp M.C., Soulier J., Tetaria C., Kaplan C. (2004). Eleven human platelet systems studied in the Vietnamese and Ma’ohis Polynesian populations. Tissue Antigens, 63: 34-40.

34. Vu Thi Kim Lien, Le Thi Kim Tuyen, Le Nhat Minh and Nguyen Hoai Giang (2004). Purification of T7 DNA used in restriction enzyme screeening. (in vietnamese). J. Sci. Technol., 42 (1): 42-46.

35. Le Thi Kim Tuyen, Combet E., Nguyen Hanh Phuc (2004). A method for

measles strain characterization based on the RFLP of the variable part of the N gen. Proceedings of the 2004th National Conference on Life Sciences, Science and Technics Publishing House, Hanoi: 487-490.
36. Bach Khanh Hoa, Tran Thi Sinh, Le Thi Kim Tuyen (2004). Phenotyping human platelet antigens of the kinh population in the center, Vietnam. (in vietnamese). J. Med. Res., 27 (1): 1-5.

37. Le Thi Kim Tuyen, Nguyen Hanh Phuc (2005).Genome analysis of measles virus isolated from vaccine by PCR-RFLP. (in vietnamese). Journal of preventive medecine, XV, 5 (76): 87-91.
38. Le Thi Kim Tuyen, Bach Khanh Hoa (2013). Lysis of recombinant Escherischia coli with lysozyme for the analysis of expressed protein. (in vietnamese). J. Med Res., 83 (3): 8-13.

39. Le Thi Kim Tuyen, Bach Khanh Hoa, Vu Nguyen Thanh, Morgan RD (2015). Expression and determination of a MmeI-like restriction enzyme found in silico (in vietnamese). J. Biotech., 13(3):831-836.
40. Nguyen Thi Mai Phuong, Le Thi Kim Tuyen, Bach Khanh Hoa, Richard D. Morgan, Tran Lien Hoa (2016). Polymorphism detection of genes isolated from natural samples alike the ORF8180 gene, member of the HauI family (in vietnamese). Vietnam Med. J., 443: 60-64.

41. Le Thi Kim Tuyen, Nguyen Thi Phuong Mai (2017). Recombination of a novel restriction enzyme using a Mae843ORF8180 - like gene isolated from natural samples (in vietnamese). Vietnam Journal of preventive medecine, 27(2): 170-176.

42. Le Thi Kim Tuyen (2017). Restriction – modification system IIG allows engineering of new protein specificities. (in vietnamese). Journal of communauty medecine, 37: 135-140.
43. Le Thi Kim Tuyen, Bach Khanh Hoa (2018). A new method to obtain expressed recombinant proteins from environmental DNA genes alike a target in silico gene. Journal of communauty medecine, 43: 82-86.